Are technical replicates a necessity in RT-qPCR? – Find out in your latest issue of BioTechniques

In this issue of BioTechniques, we present the development of a BRET-based senor for the detection and quantification of interactions involving regulatory subunit B56α, an assay for monitoring mitophagy flux in live muscle cells and a method for determining the relationship between cellular RNA and protein expression levels in bronchoalveolar lavage samples. Reports Dual compartment utility of BRET-based biosensors for PPP2R5A/B56α, a cancer-associated B regulatory subunit of PP2A Mito-Kaede photoactivation and chase experiment for mitophagy: mitophagy flux response toward various stimulations Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage Assessing the necessity of technical replicates in reverse transcription quantitative PCR An enzyme-free alcohol-based organoid harvesting solution If you missed last month’s issue, catch up here >>> Get this straight to your inbox each month by signing up for Journal updates >>> The post Are technical replicates a necessity in RT-qPCR? – Find out in your latest issue of BioTechniques appeared first on BioTechniques.